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KMID : 0861120120160010043
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Korean Journal of Oriental Preventive Medicine
2012 Volume.16 No. 1 p.43 ~ p.56
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The Anti-bacterial Effects and Epidermal Permeability Barrier Function of Red Onion Juice Produced in Jeon-Nam province in Korea
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Youn Dae-Hwan
Shin Heon-Tae
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Abstract
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Object: In this study, we assessed the anti-bacterial effects and epidermal permeability barrier function of red onion juice comparing to yellow onion juice and extract .
Methods: 3types of red and yellow onion juice were prepared as antibacterial agent candidates with Houttuynia cordata hot water extract using 4 different bacterial strains ( , , , ) by colony counting method. The expression of filaggrin, a marker of keratinocyte differentiation, and serine palmitoyl transferase (SPT), a marker of the formation of the stratum corneum lipid barrier, in human HaCat keratinocytes were analyzed using HaCaT cell line. The expression of COX-2 and AP-1 which is a factor of COX-2 transcription were also analyzed by western blotting method.
Results: There was detectable anti-bacterial effects on , among 1%, 5%, 10% extracts of yellow and red onion.(81%-100%) The bacteriocidal effects were not shown on , among , yellow onion and red onion extracts. The in vitro results showed the concentration-dependent effects on the expression of both filaggrin and SPT in HaCat cells among 0.01%, 0.05%, 0.1%, 0.5% extracts in Houttuynia cordata and red onion, reflecting the notion that and red onion can induce epidermal keratinocyte differentiation and improve the recovery of skin barrier functions. The concentration-dependent effects also have been shown on the expression of both COX-2 and AP-1 among 0.01%, 0.05%, 0.1%, 0.5% extracts in and red onion, while slight effect in yellow onion.
Conclusion: Red onion juice could be a potential candidate enhanser for the skin care and cosmetology.
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KEYWORD
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anti-bacterial, Houttuynia cordata, red onion, filaggrin, serine palmitoyltransferase (SPT), Cox-2, AP-1
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